1 October 2011 (Saturday) - ALL? - AML !

Patient presented at GP with abdo pain and lack of appetite.



MOUSE,MICKEY                         A+        22/09/2011 u/k
02/02/1951   Male    696969          ITU
                     abdopain not eating drinking..                                              U         LONC
Specimen No   :  AK672862H               Selected Auth Level : E

 HB     13.5   S000 MONO   2.6    S000
 WBC    7.4    S000 EOS    0.4    S000
 PLT    44     S000 BASO   0.1    S000
 RBC    4.52   S000
 HCT    0.415  S000
 MCV    91.8   S000
 MCH    29.9   S000
 MCHC   32.5   S000
 NEUH   0.3    S000
 LYMPH  4.1    S000



The   biochemistry showed:

Sodium           137   mmol/L       (133 to 146) Auth
Potassium         4.1   mmol/L       (3.5 to 5.3) Auth
Creatinine        274   umol/L       ( 64 to 104) Auth
GFR (estimated)   20    units=*                   Auth
Total Protein     70    g/L          ( 60 to 80 ) Auth
Albumin           33    g/L          ( 35 to 50 ) Auth
Total Bilirubin   27    umol/L       (  0 to 29 ) Auth
Alk Phos          324   U/L          ( 30 to 130) Auth
ALT               25    U/L          (  0 to 70 ) Auth
Calcium           2.3   mmol/L       (2.2 to 2.6) Auth
Alb-cor calcium   2.4   mmol/L       (2.2 to 2.6) Auth
Urate             649   umol/L       (200 to 430) Auth
CRP               122   mg/L         (  0 to 10 ) Auth


The blood film showed blast cells, and the case was referred to consultant haematologist
 
CIRCULATING LYMPHOBLASTS . THROMBOCYTOPENIA . NO VISIBLE PLATELET CLUMPS.
NEUTROPENIA. COULD BE NEWLY PRESENTING ALL. MEDICAL TEAM
INFORMED URGENTLY
Subsequent investigations showed:

Coag:

PT             16.4 s            (12 to 16)     Auth
APTT           39.8 s            (22.0 to 35)   Auth
Fibrinogen     5.44 g/L          (1.90 to 4.30) Auth
Thrombin Time  16.9 s            (13 to 20)     Auth
Reptilase time 17.3 s            (14 to 19)     Auth



Serum Total protein  70 g/L          (60 to 80) Auth
Serum Albumin        33 g/L          (35 to 50) Auth
Globulin             37 g/L          (20 to 35) Auth
Electrophoresis           showed the following:                          Auth
    Comments :
     Increased alpha 1 & 2 globulins
     Slight polyclonal increase in gammaglobulins.
     This electrophoretic pattern is consistent with an acute phase
     response.



 Bone marrow was aspirated and scrutinised:

Bone Marrow Investigation:

Report Sequence No.1     22/09/11   09:50     Status : S000

BONE MARROW ASPIRATE AND TREPHINE FROM RPIC EASILY OBTAINED

APARTICULATE HYPERCELLULAR TRAILS
MEGAKARYOCYTES PRESENT WITH SOME HYPOLOBATED FORMS PRESENT.

DIFFERENTIAL BLASTS 80% PRO 1% META 1% ,MYELO 1%
NEUTROPHILS 3% LYMPHOCYTES 4% EOSONOPHILS 2%  ERYTHROID
8%

ERYTHROPOIEISIS AND GRANULOPOIEISIS MARKEDLY REDUCED AN EXCESS OF LYMPHOBLASTS PRESENT ACCOUNTING FOR 80% OF CELLS.

CONSISTENT WITH ACUTE LYMPHOBLASTIC LEUKAEMIA

Reference Laboratory reported on Immunophenotyping and bone marrow:


Results from a gate representing approx. 97% of Total Nucleated Cells (TNC’s):

Immunophenotyping demonstrates a CD34+ cell population representing approx 48% TNC’s with a profile typical of myeloid progenitors (CD13+, CD33-, 1/2CDCD117+, 1/2HLA+, MPO+ and LYSO-).




BONE MARROW ASPIRATE AND TREPHINE EASILY OBTAINED FROM
RPIC

OLIGOPARTICULATE HYPERCELLULAR PARTICLES AND TRAILS VERY FEW MEGAKARYOCYTES SEEN AND HYPOLOBATED

DIFFERENTIAL: BLASTS 80% PROMYELOCYTES 9% MYELOCYTES 5% ERYTHROID 6%

AN EXCESS OF MYELOBLASTS OF TYPE 1 MORPHOLOGY SEEN WITH MARKEDLY REDUCE ERYTHROPOIEISIS AND GRANULOPOIESIS. CONSISTENT WITH A DIAGNOSIS OF ACUTE MYELOID LEUKAEMIA

VERIFIED BY HAEM MDM



The final word on the diagnosis came from the reference laboratory:


Consultant Comments: Type 1 Blasts account for over 90% of TNC’s. Type 2 blasts occasionally seen. No Auer Rods. Blasts are medium-large sized. Consistent with AML (M1) by morphology and Immunophenotyping.


So the final diagnosis was AML (M1). The moral of this story is that in acute leukaemias, the morphology can be misleading. Consultant medical staff can be unsure on the differentiation between myeloblast and lymphoblast. The final diagnosis must be made by flow cytometry which looks for specific cell markers.

 

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