11 March 2025 (Tuesday) - NEQAS 2501DM

I got the results of NEQAS 2501DM today.
 
You are asked your opinion of this blood film that was prepared from a 64-year old female who had attended her GP complaining of tiredness. The automated analyser had reported a lymphocytosis with a flag indicating the possible presence of blast cells”.
 
I saw
 
Rbc
 
Rouleaux
 
Wbc
 
Lymphocytes with blebbing
Lymmphocytes with Burkett-like vacuoles
Smear cells
Blast cells
Dysplastic neutrophils
Toxic granulation
 
Plt
 
Giant platelets
 
I felt this was something lymphoproliferative.
 
The expert opinion said ”The abnormal cells are variable. However, most cells have a mature appearance, and the preservation of neutrophil and platelet numbers makes a diagnosis of acute leukaemia less likely. Furthermore, the overall nuclear and cytoplasmic features are not typical of either circulating blast cells. Making a morphological diagnosis of MCL can be difficult and we can rarely (if ever) be confident of the diagnosis using morphology alone. However, MCL is a potentially aggressive disorder, so it is important not to simply report the appearances as reactive. Features to look for include – marked variability between malignant cells with some cells looking quite mature and others more aggressive; a variable but often abundant and basophilic cytoplasm; and nuclear complexity, classically indented but often quite variable. Confirmation can then be made using marker studies and molecular testing.
 
Which (quite frankly) means that I’d spotted the salient features and gone about as far as I could with a microscope. I’ll take that as a success.

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