13 October 2015 (Tuesday) - BT NEQAS Report
There was a rather interesting discussion on the report of the latest blood transfusion NEQAS report:
“Where antibody identification cannot be concluded using the patient phenotype and results of testing all available panel cells by IAT and enzyme, additional techniques can be used to make progress. E.g. the presence of anti-M can be excluded or confirmed using an antibody panel by direct agglutination at room temperature.
Although anti-K would normally be expected to react in a 2-stage enzyme technique, it is advisable to exclude its presence by IAT, since the sensitivity of the 2-stage enzyme technique can be variable.
Positive identification requires at least two positive reactions with red cells that express the corresponding antigen (and are antigen negative for other specificities potentially present), as a single positive reaction could be a false positive or
be due to an antibody to a low frequency antigen
When interpreting antibody identification results, all available information should be reviewed, including results of all techniques and of the screening panel1, to ensure that all possible exclusions are made and that there are no reactions unaccounted for by the antibodies identified.”
I need to think outside the box a little more in cases like this…